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Cell lysates from 3D culture were collected and protein concentration in each sample was measured by the BCA assay (Thermo Scientific). Three hundred milligrams of protein were analyzed using the Human Phospho-Receptor Tyrosine Kinase Array Kit (R&D Systems) according to the manufacturer’s protocol. After applying chemiluminescence detection solution, membranes were exposed to imaging film and developed using a Kodak X-Omat processor (Kodak).
Copyright and License information: ©2017
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