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Autophagy analyses by confocal microscopy
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Forty-eight hours after transfection of miR-18a-5p mimic and pGTLV-EGFP-mRFP-LC3 virus, counted the GFP-LC3 dot total number of transfected cells. The cells observed under the Laser Scanning Confocal Microscopy (Occult International Ltd, Germany) counted GFP-LC3 dot positive cells number.
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Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
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