In vivo culture of Trichuris muris

Severe combined immune deficiency (SCID) mice were bred in the Biological Services Facility at the University of Manchester. Both male and female SCID mice were infected with a high dose of 200 infective embryonated T. muris eggs via oral gavage. At day 35 post-infection, groups of 5 mice were killed and their caecae and colons removed. Guts were opened longitudinally, washed with pre-warmed Roswell Park Memorial Institute (RPMI) 1640 media supplemented with penicillin (500U/ml) and streptomycin (500μg/ml) and adult T. muris worms gently removed using fine forceps.