In vitro CK2 kinase assay

NN Nadine Nelson
KS Karoly Szekeres
CI Cristina Iclozan
IR Ivannie Ortiz Rivera
AM Andrew McGill
GJ Gbemisola Johnson
ON Onyekachi Nwogu
TG Tomar Ghansah
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CK2 kinase activity was measured in splenocytes from CTRL, TB and TB-API mice using the CK2 assay kit (Millipore) according to the manufacturer’s instructions [36]. CK2 activity was calculated by subtracting the mean counts per minute (CPM) of samples in the absence of substrate from the mean CPM of samples in the presence of substrate [36].

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