Rhodamine 123 efflux assay

HS Hye-Sook Seo
JK Jin Mo Ku
HC Hyeong Sim Choi
JW Jong-Kyu Woo
BL Byung Hoon Lee
DK Doh Sun Kim
HS Hyun Jong Song
BJ Bo-Hyoung Jang
YS Yong Cheol Shin
SK Seong-Gyu Ko
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MCF-7 and MCF-7/ADR cells were treated with apigenin (0–80 µM) or nicardipine (positive control, 12.5 µM) for 24 h and incubated for another 1 h with 1 µg/ml of rhodamine 123. Accumulation of rhodamine 123 in the cells was analyzed by flow cytometry.

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