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Lumbar puncture was performed after participants had fasted overnight. A neurologist (P. R-N.) inserted a sterile 24 gauge Sprotte atraumatic spinal needle in the L4-L5 interspace to withdraw 29 ml of CSF (yielding clinical lab samples and 25 0.5 ml aliquots). The latter were quick-frozen on dry ice and stored thereafter at -80C. CSF proteins were analyzed via ELISA using the Innogenetics Innotest kits for Aβ42, total-tau, and P-tau.
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