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Statistical analyses were performed using SPSS for Windows, version 15.0 (SPSS Inc., Chicago, IL, USA). For calculations of statistical significance, two-sided testing was used and p < 0.05 was considered as significant. One-factor analysis of variance (ANOVA) was applied for data comparison between multiple groups and the Tukey post-hoc test was applied to determine the relationships between the groups. The Student’s t-test was applied when only two groups were compared.

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