Cell lines and transfections

Ba/F3 cells were obtained from the RIKEN BRC CELL BANK (Ibaraki, Japan) and cultured in RPMI 1640 supplemented with 10% fetal bovine serum and 1 ng/mL recombinant IL3 (R&D Systems). Then, 5 × 10⁶ cells were electroporated with 2.5 mg pcDNA3.1/FGFR2-constructs, pcDNA3.1/FGFR2-ACSL5 fusion constructs, or parental pcDNA3.1, using the Nucleofector system (Lonza, Basel, Switzerland). The cells were selected in medium containing G418 for 2 weeks and the expression of the full-length fusion transcripts was confirmed by immunoblotting. For detection of cell viability, 5 × 10³ Ba/F3 cells per well were plated in quadruplicate in 96-well plates with various concentrations of IL3 or AZD4547. After 72 hours, cell viability was determined using CellTiter-Glo.