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WESTERN BLOT ANALYSIS

MZ Michael Zimmer
PB Pradeep Bista
EB Ericka L. Benson
DL Diana Y. Lee
FL Feng Liu
DP Dominic Picarella
RV Rick B. Vega
CV Chi B. Vu
MY Maisy Yeager
MD Min Ding
GL Guosheng Liang
JH Jay D. Horton
RK Robert Kleemann
TK Teake Kooistra
MM Martine C. Morrison
PW Peter Y. Wielinga
JM Jill C. Milne
MJ Michael R. Jirousek
AN Andrew J. Nichols
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For HuH‐7 cell experiments, whole‐cell lysates were prepared and subjected to immunoblot analyses of SREBP‐1 and SREBP‐2 as described.23 For HepG2 experiments, cells were lysed in radio immunoprecipitation assay buffer with protease and phosphatase inhibitors. Densitometry was performed relative to actin in matched lanes. Goat anti‐human SREBP‐2 antibody was from R&D Systems (AF7119). LDLR antibody was from BioVision (3839‐100). Beta‐actin antibody was from Abcam (Ab8226). Secondary antibodies were from Li‐Cor.

Copyright and License information:  ©2017 The Authors. Hepatology Communications published by Wiley Periodicals, Inc., on behalf of the American Association for the Study of Liver Diseases.
This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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