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Radioimmunoassay

AD A. T. Desaulniers
RC R. A. Cederberg
GM G. A. Mills
CL C. A. Lents
BW B. R. White
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Unextracted serum was analyzed in duplicate to quantify concentrations of testosterone by a double antibody radioimmunoassay (RIA) kit (cat # 07-189102; MP Biomedicals, Aurora, OH), in accordance with manufacturer’s instructions. Additional standards (0.03 and 0.16 ng/ml) were added to reach the sensitivity of the assay (0.03 ng/ml). The intra- and inter-assay coefficients of variation (CV) were 6.5 and 7.7%, respectively. Concentrations of LH were determined by RIA at the Colorado State University Reproductive Endocrinology Laboratory (Fort Collins, CO). The intra- and inter-assay CV were 2.7 and 6.2%, respectively.

Copyright and License information: The Author(s) ©2017
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