Acute anti-nociceptive activity using hot-plate test

The hot-plate test was performed according to methods described by Hosseinzadeh et al (11). The hot-plate temperature was fixed on 55 °C and cut off time was 20 sec. Jumping, withdrawal of paws and/or licking of paws were counted as positive response to stimuli. 6 male mice were used for each group of tests. Mice responses were determined at the time of injection (time 0) and after 30, 60 and 90 min of administration (normal saline, QC 0.937, 2.812 and 6.562 mg/kg and morphine 10 mg/kg).