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Measurement of inflammatory cytokines
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Serum levels of the inflammatory cytokines murine IL-1β, IL-6, interferon (IFN)-γ, TNF-α, and IL-10 were determined using the Luminex multiplex cytokine assay (Luminex 200 system, Millipore, Billerica, MA, USA) according to the manufacturer’s recommendations. The level of TGFβ1 was measured using a commercially available mouse TGFβ1 enzyme-linked immunosorbent assay (ELISA) kit (R&D Systems).
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