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Protein synthesis analysis
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Puromycin (Millipore Sigma) was added to cell culture medium to a final concentration of 1 µM and incubated for 30 min as previously described (Goodman et al., 2011). At that dose, puromycin incorporation into neosynthesized proteins reflects the rate of mRNA translation in vitro (Schmidt et al., 2009). Puromycin incorporation was detected by Western blot analysis.
Copyright and License information: ©2017 Neppl et al.
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