Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Concise Method
Measurement of Urinary Protein and Nitrate/Nitrite
Ask a
question
Favorite
Urine collected at harvest was used to assess total protein and total nitrate/nitrite. Total protein levels were determined using urine diluted 1:5 by the bicinchoninic assay (Pierce) according to the manufacturer's instructions. A commercially available nitrate/nitrite colorimetric assay kit (Cayman Chemical 780001, Ann Arbor, MI) was used to assess total nitrate/nitrite concentrations. Urine (80 μL) from each rat diluted 1:10 was assayed in duplicate according to the manufacturer's instructions.
Copyright and License information: ©2017 The Authors. Published on behalf of the American Heart Association, Inc., by Wiley.
This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
