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Lentivirus preparation and infection
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Lentiviral packaging was carried out in HEK-293T cells using a packaging system from SBI per the manufacturer's protocol, as described previously [33]. For infection, exponentially growing cells were mixed with viral particles in the presence of polybrene (0.8 mg/ml) in a six-well plate at a multiplicity of infection of 1∼3.
Copyright and License information: ©2016 Zhang et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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