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Evaluation of oocytes following IVM
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At the end of IVM, the COCs were examined for cumulus cell expansion (CCE). The cumulus cells were removed by incubating them with 100 IU/ml of hyaluronidase for 15 min at 37°C and repeatedly passing them through a fire polished narrow bore glass capillary. The denuded oocytes were examined under an inverted microscope (TH4-200, Olympus, Japan) for the presence of the 1st polar body (PB) in the perivitelline space.
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