Fluorescence-Activated Cell Sorter Analysis and Imaging of HMGCR-mNeon

Cells were treated as indicated and analyzed on a CytoFLEX Flow cytometer (Beckman Coulter). Viable cells were gated and 10 000 events per condition acquired and analyzed using the FlowJo software package. Relative HMGCR-mNeon intensity was calculated from GEOmean values and presented as mean±SD. For confocal imaging, Hap1-HMGCR-mNeon cells were fixed with 4% paraformaldehyde for 10 minutes and viewed with A Leica TCS SP8 confocal microscope equipped with a ×63 objective. For live cell imaging, we used a Leica IR-BE inverted wide field microscope equipped with a ×63 objective and a custom-built incubator.