GC–MS analysis of cypermethrin biodegradation

To check the degradation of cypermethrin in soil slurry, 50 g autoclaved soil was taken in a 100-ml flask, to which 20 ml of minimal medium was added. Cypermethrin (100 ppm) was added to all the sets, to which 1 ml of 24-h-old bacterial culture was added and mixed properly. One ml of soil slurry was taken from all the flasks separately on 0, 10 and 15th day after incubation. Uninoculated flasks spiked with pesticides acted as control. Extraction and quantification of cypermethrin was done by HPLC. For GC–MS analysis, samples were extracted from soil slurry and metabolites of biodegraded cypermethrin were identified at AIRF facility, JNU, New Delhi.