Reagents

Zoledronic acid (zoledronate or Zol) monohydrate, alendronic acid (alendronate or Ale) and disodium clodronic acid (clodronate or Clo) tetrahydrate were from TCI America (Portland, OR). Alexa Fluor 647 (AF647)-labeled Zol was from BioVinc LLC (Los Angeles, CA). Sodium hydrate, calcium chloride, zinc chloride, sodium dihydrogen phosphate, sodium hydrogen phosphate, uranyl acetate (UA) dihydrate, formic acid, formalin, collagenase IV, paraformaldehyde, polyoxymethylene, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), polyoxyethylene (5) nonylphenylether (NP-5) and polyoxyethylene (10) octylphenyl ether (OEP-10) were from Sigma-Aldrich (St. Louis, MO). DOPA monosodium salt and 1, 2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy (polyethylene glycol)-2000] (DSPE-PEG_2K) were from Avanti Polar Lipid, Inc. (Alabaster, AL). Cyclohexane, dimethyl sulfoxide, hexanol, chloroform and ethanol were from Thermo Fisher Scientific Co. (Pittsburgh, PA). APC-labeled anti-mouse CD206 (MMR) antibody was from BioLegend Co. (San Diego, CA). All reagents were used without further purification.