Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

RNA extraction and qRT-PCR for cellular mRNAs.

PL Priya Luthra
SA Sebastian Aguirre
BY Benjamin C. Yen
CP Colette A. Pietzsch
MS Maria T. Sanchez-Aparicio
BT Bersabeh Tigabu
LM Lorraine K. Morlock
AG Adolfo García-Sastre
DL Daisy W. Leung
NW Noelle S. Williams
AF Ana Fernandez-Sesma
AB Alexander Bukreyev
CB Christopher F. Basler
request Request a Protocol
ask Ask a question
Favorite

The total RNA was isolated from the cells using Trizol according to the manufacturer’s instructions. cDNAs were synthesized using the SuperScript III First-Strand synthesis system (Invitrogen). The resulting cDNAs were used as the templates for subsequent quantitative PCRs using gene-specific primers (IFN-β, ISG54, IFN-α, or β-actin).

Copyright and License information:  ©2017 Luthra et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A