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Myocardial cell apoptosis was evaluated by TUNEL method with the In situ Cell Death Detection Kit (Roche, Indianapolis, USA) according to the manufacturer's instructions. After TUNEL staining, myocardial cells were stained with DAPI (Biosharp, China) solution for 20 min at room temperature. The samples were observed under fluorescence microscope (Confocal microscopy Carl Zeiss LSM700).
Copyright and License information: ©2017 Liu et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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