Kcne3, Kcnn4 and Kcnk5 null and Cftr‐ΔF508 mutant mice

Kcne3 KO animals have been generated by deletion of exon 4 that contains the whole coding region of the Kcne3 gene (Preston et al. 2010). Kcnn4 KO mice were those generated by Begenisich et al. (2004). The Kcnk5 KO mouse was generated using a trapping vector encoding a β‐galactosidase (Taniwaki et al. 2005) and lacks expression of K_2P channel TASK‐2. The Cftr^tm1Eur mouse (van Doorninck et al. 1995) carries the mutant ΔF508 of the CFTR protein.

Only male mice were used throughout, except for the Cftr^tm1Eur mice for which three males and three females were used.