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Two-Electrode Voltage-Clamp Recording.
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Xenopus laevis oocytes were used to express cloned rat or human nAChR subtypes heterologously. Recordings were made 1–5 d postinjection. Oocytes were voltage-clamped at −70 mV at room temperature and exposed to acetylcholine, RgIA, or analogs as described previously (20).
Copyright and License information: ©2017
Freely available online through the PNAS open access option.
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