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Cell proliferation was assayed using a cell proliferation kit (MTS) (Promega, Madison, WI) according to the manufacturer's protocol. Cells transfected were seeded into 96-well plates at a density of 3×103 cells/well in 100μl medium. Cell growth proliferation was assessed every 24 h according to the manufacturer's instructions. 20μl of MTS regent was added to each well and incubated at 37 °C for 2 h. Absorbance was recorded at 490 nm with Envision microplate reader (PerkinElmer). Experiments were performed in triplicate.
Copyright and License information: ©2017 Pei et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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