Cell viability and apoptosis assays

Cells were seeded at 20,000 cells/well in 96-well plates. Test compounds were added in quadruplicate using the HP D300 Digital Dispenser (Hewlett Packard Inc.). Inhibition of cell viability was assessed after 96 hours using CellTiter Glo^® Luminescent Cell Viability assay (Promega Corp., Madison, WI). Data was plotted as percent of vehicle (DMSO) control using GraphPad Prism (San Diego, CA). For apoptosis assays, cells were seeded at 0.2 X 10⁶/mL in a 12 well dish. Apoptosis was measured after 48 hours using FITC Annexin V Apoptosis Detection Kit II or PE Annexin V Apoptosis Detection Kit I (BD Biosciences, San Jose, CA) following manufacturer's protocol. Labeled cells were measured by flow cytometry using BD LSRII and analyzed using FACSDiva^™ (BD Biosciences).