2.3. Induction of Topical AD-Like Skin Dermatitis in Mice by Oxazolone and Treatment with DQ

4-Ethoxymethylene-2-phenyl-2-oxazolin-5-one (oxazolone) (1%) dissolved in vehicle (propylene glycol:EtOH = 7:3) was used as sensitizer to induce AD in BALB/c mouse ears according to the previously described method [20]. In brief, the ears of BALB/c mice were sensitized with 20 μL of 1% oxazolone on the first day. After the first challenge, 20 μL of 0.1% oxazolone was repeatedly applied to ears for an additional 3 weeks at 2-day intervals. At the same time, the ears of the BALB/c mice were exposed to 20 μL of 1% DQ daily in the oxazolone-DQ-group for 3 weeks, and the application of 1% DQ was separated by 4 h from that of oxazolone. The normal control animals (CON) were treated with distilled water alone. No substances were applied to the skin surface on the last day of the experiment. On the last day, measurements of skin inflammation signs, including ear swelling and erythema, were carried out.