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SIRT1 deacetylation activity was measured using a SIRT1 Activity Assay Kit (Fluorometric) (Abcam) as described previously [16] and analyzed in a microplate reader according to the manufacturer's guidelines. For this experiment, SIRT1 protein was concentrated by immunoprecipitation using protein A agarose beads (Santa Cruz, Dallas, TX, USA). OvCa cells with either endogenous low SIRT1 mRNA expression (ES2 cells), or high SIRT1 mRNA expression, with known cisplatin resistance (A2780CDDP cells) were selected for analyzing SIRT1 activity.
Copyright and License information: ©2017 The Authors
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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