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Blood collection and experimental parameters
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After 4 weeks of ALA treatment, the rats were fasted overnight for 12 h and blood sampling was done from the lateral saphenous vein under a conscious condition in all groups of rats. The blood samples were either immediately subjected to biochemical analysis or were allowed to clot for 30 minutes before being centrifuged at 850g for 15 min. The serum was separated and stored at −20°C until biochemical analysis.
Copyright and License information: ©2017 The Authors. published by John Wiley & Sons Ltd, British Pharmacological Society and American Society for Pharmacology and Experimental Therapeutics.
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