Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Concise Method
Assessment of the immunogenic cell death pathway
Request a Protocol
Ask a
question
Favorite
The release of ATP from vesicles were quantitated using a commercially available CellTiter-Glo Luminescent assay for ATP (Promega, Madison, Wisconsin, USA), following the protocol provided by the manufacturer.
The release of HMGB1 into cell supernatants was quantitated by an enzyme-linked immunosorbent assay (HMGB1 ELISA kit II, Shino Test Corporation, Tokyo, Japan), following the manufacturer's instructions.
Copyright and License information: ©2017 Han et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
