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Cytospin

ER Emma C. Reilly
KL Kris Lambert-Emo
DT David J. Topham
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A glass slide and a single Cytofunnel were placed into a Cytoclip for each sample (Thermo Scientific Shandon). 3x104 cells were added in a volume of 100μL PBS to each funnel. Clips were placed in the Cytospin 2 (Thermo Scientific Shandon) and run at 1,000rpm for 5 minutes. Slides were removed and allowed to dry at room temperature. Diff Quick stain was used to stain slides (Siemens) prior to counting on a light microscope.

Copyright and License information:  ©2016 Reilly et al
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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