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BCBL1 or HKB5/B5 cells were transfected with a fixed amount of plasmid DNA. The reporter assays were carried out according to manufacturer's protocol for the luciferase reporter assay system (Promega). The fold activation of each reporter construct was calculated as the luciferase activity in the presence of stimuli (including high glucose) divided by that in the absence of stimuli.
Copyright and License information: ©2017 Chang et al.
This article is distributed under the terms of the Creative Commons Attribution License (CC-BY), which permits unrestricted use and redistribution provided that the original author and source are credited.
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