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Assays were performed as described previously.58, 59, 60 Briefly, 10 000 cells per well of Molt-4 or Jurkat cells in log phase growth or 100 000 cells per well of PBMCs were added to 96-well plates in 100 μl in the presence of test compounds and fresh media. Cells were cultured for 72 h. During the last 2 h, cells were labeled with 10 μl of CellQuantiBlue reagent, and were scanned with a Victor PerkinElmer (Waltham, MA, USA) plate reader (ex550/em600).
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Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
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