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Active Ras pull down and detection assay
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Cell lysates were collected and assayed by the active Ras pull-down and detection kit (Thermo Scientific, Waltham, MA). Briefly, the GTP-form of Ras was pulled down by a GST-fusion protein with the Ras-binding domain (RBD) of Raf attached to glutathione agarose. The pull-down complexes were washed and separated on a 10% SDS-PAGE gel and immunoblotted with an anti-pan-Ras antibody.
Copyright and License information: ©2017 Ganapathy et al.
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