The leukemic cells were incubated in culture medium, with or without (the control) drug treatment. The fractions of viable and apoptotic cells following 48 h of exposure were determined by Vybrant Apoptosis assay kit #4 (Molecular Probes; Thermo Fisher Scientific, Inc., Waltham, MA, USA), as described previously (15).
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.