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Protein A beads were co-incubated overnight at 4°C with A431 lysates and pre-immune or immune sera. As positive controls for HER1 and HER2 precipitation respectively, 2μg of nimotuzumab and trastuzumab were used instead serum. The precipitated was extensively washed, applied to 7.5% SDS-PAGE, transferred to PVDF membranes and immunobloting was performed to detect HER2 and HER1 with specific antibodies as described.
Copyright and License information: ©2017 González Suárez et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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