Rodent PK.

Pharmacokinetic (PK) studies were carried out with infected female CD-1 mice in the in vivo bacteremia infection study described above, following i.v. and oral dosing at 8 mg/kg and 50 mg/kg. A PK study was also conducted with normal male Wistar Han rats with an i.v. dose of 5 mg/kg and an oral dose of 10 mg/kg. AM-8722 was formulated in 30% (wt/wt) Captisol in water for both i.v. and p.o. administration. Following dosing of animals, blood collection was performed at predetermined time points. Serial mouse blood samples (20 μl) from the tail vein were added to 60 μl of 0.1 M trisodium citrate buffer and stored at −80°C until liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. Rat blood was collected in EDTA tubes, and rat plasma was then obtained by centrifugation of the blood. Mouse whole blood and rat plasma samples were analyzed by LC-MS/MS to quantify AM-8722 concentrations. Pharmacokinetic parameters of AM-8722 were calculated using Watson LIMS software (version 7.4.1; Thermo Fisher Scientific, Philadelphia, PA).