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Analysis of mutant phenotypes

PK Pawel Z. Kosentka
LZ Liang Zhang
YS Yonas A. Simon
BS Binita Satpathy
RM Richard Maradiaga
OM Omar Mitoubsi
ES Elena D. Shpak
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Measurements of stomata index and clustering were done on the abaxial side of cotyledons from 17-d-old seedlings using differential interference contrast (DIC) microscopy. For DIC, seedlings were incubated in a solution of 9:1 ethanol:acetic acid overnight, rehydrated with an ethanol series to 50% (v/v) ethanol, and then cleared in a mixture of 8:1:1 chloral hydrate:distilled water:glycerol.

Copyright and License information:  ©2017-02-15 The Author 2017. Published by Oxford University Press on behalf of the Society for Experimental Biology.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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