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The PBS-rinsed 48 h biofilm on the disk stained using the BacLight live/dead bacterial viability kit (Molecular Probes, Eugene, OR, USA). Live bacteria cells were stained with SYTO 9 emitting green fluorescence, while dead cells were stained with propidium iodide emitting red fluorescence. Biofilms of each group were examined by confocal laser scanning microscopy (Leica, Wetzlar, Germany).
Copyright and License information: ©2017 by the authors.
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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