Scratch Motility Assay

Scratch motility assays were performed by growing cells to confluency in a 6-well plate. Cells were starved in serum-free media (SFM) supplemented with mitomycin C two hours prior to the start of the assay. In each well three T-shaped wounds were induced with a p200 tip. Images were captured at baseline (0 h) and at the endpoints indicated: 48 h (MCF-7) and 36 h (MB-231). The percent open image area for each T-shaped wound was measured using TScratch Software ⁽⁴⁰⁾. This assay was repeated in triplicate.