Cell viability assays using ATP measurement

Cell Titer Glo (Promega) was used for cell viability estimation. Cells were plated at a density of 5,000∼10,000 cells per well in 100 μL and cultured for 48 or 72 hours with or without treatment. The plates were then removed from the incubator and allowed to equilibrate to room temperature for about 10 minutes. Cell Titer Glo solution was added at 100 μl per well, and the plates were kept in the dark for 15 minutes before the luminescence was read by luminometer (Luminoskan Ascent; Thermo Scientific Corporation).