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Protein samples were prepared by precipitation with acetone followed by pellet drying and resuspension in sample buffer. Staining was carried out with Biosafe® Coomassie for protein detection and barium iodide for PEG detection. The SDS-PAGE gels were scanned using a ChemiDoc® imager (Biorad) and relative bands intensity was evaluated by densitometric analysis. At least two different protein concentrations were tested to verify that sample loading was within linearity range.
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