Cisplatin-Induced Acute Kidney Injury Mouse Model

Cas9 mice (males and females, 8 to 12 weeks old) received an intraperitoneal injection of 15 mg/kg cisplatin (Tocris Bioscience, Ellisville, Missouri) 8 days after tail vein injection of AAV. Kidney tissues and blood serum samples were collected 4 days after cisplatin administration. Blood serum was assayed for blood urea nitrogen (BUN) and serum creatinine (S-Cre) levels using commercially available assays (QuantiChrom Urea Assay Kit and QuaintChrom Creatinine Assay Kit; BioAssay Systems, Hayward, CA) as renal function parameters. Collected kidney samples were fixed in 4% paraformaldehyde (PFA) and embedded in OCT compound (Sakura Tissue-Tek) after PBS wash and quickly frozen in ethanol. Cryostat sections (10 µm) were stained with either hematoxylin and eosin (H&E) or periodic acid-Schiff’s reagent (PAS). Tubular necrosis, urinary casts, tubular dilation, and tubular borders were assessed in non-overlapping fields (high power field) as described (Imberti et al., 2015; Li et al., 2016).