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Sanger sequencing

CL Chaoyue Liang
ZW Zhuolin Wu
XG Xiaohong Gan
YL Yuanbin Liu
YY You You
CL Chenxian Liu
CZ Chengzhi Zhou
YL Ying Liang
HM Haiyun Mo
AC Allen M. Chen
JZ Jiexia Zhang
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Genomic DNA was amplified with four primer pairs targeting exons 18 to 21 and labeled using the EGFR Mutation Detection Kit (Guangzhou Life Technologies Daan Diagnostics Co., Ltd., Guangzhou, China). Sequencing and data collection were performed using an ABI 3100 Genetic Analyzer (Applied Biosystems). All sequence variations were confirmed by multiple independent PCR amplifications and repeat sequencing as previously described.12 The difference between high and low mutation abundance was as previously defined.13

Copyright and License information: Yonsei University College of Medicine ©2018
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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