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Cells were plated in 96-well plates at 2,500 cells per well in triplicate. New serum-free medium with 10 to 500 μg/ml concentration of Herceptin was added for 3 days. MTT solution (5 mg/mL in PBS, Amresco) was added to each well and cells incubated for 2 hours. Media was then aspirated and cells resuspended in DMSO. Absorbance at 560 nm was measured, with background at 670 nm subtracted. Triplicates were averaged for a mean absorbance. Percentage surviving was calculated as the absorbance of treated cells relative to time-matched vehicle-treated cells.
Copyright and License information: ©2017 Park et al.
This article is distributed under the terms of the Creative Commons Attribution License (CC-BY), which permits unrestricted use and redistribution provided that the original author and source are credited.
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