H1975 NSCLC xenograft model

Female NCI Ath/nu mice were implanted with H1975 NSC lung adenocarcinoma model. Once tumors reached an average size of 100- 150 mm³, mice were randomized into treatment cohorts. Mice were orally administered daily afatinib (20 mg/kg, Genentech/OSI), erlotinib (70 mg/kg, LC Labs), or G1T38 (50 or 100 mg/kg), as single agents or in combination (G1T38 + erlotinib or G1T38 + afatinib) for the duration of the study. Tumors were measured twice weekly until mice reached tumor burden of 1500 mm³. All protocols were IACUC approved and experiments were completed at Charles River Laboratories (CRL; Research Triangle Park, NC).