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Nanosyn CDK biochemical in vitro assay
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Compounds were tested in CDK1/CYCLIN B1, CDK2/CYCLIN A, CDK2/CYCLIN E, CDK4/CYCLIN D1, CDK6/CYCLIN D3, CDK5/p25, CDK5/p35, CDK7/CYCLIN H-MAT1, and CDK9/CYCLIN T kinase assays (Nanosyn, Inc.; Santa Clara, CA). The assays were completed using microfluidic kinase detection technology (Caliper Assay Platform). The compounds were tested in 12-point dose response format in singlicate at the Km for ATP. Phosphoacceptor substrate peptide concentration used was 1 μM and Staurosporine was used as the reference compound for all assays.
Copyright and License information: ©2017 Bisi et al.
This article is distributed under the terms of the Creative Commons Attribution License (CC-BY), which permits unrestricted use and redistribution provided that the original author and source are credited.
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