4.12. Assessment of Cancer Cell Anti-Proliferative Activity Using the MTT Cell Viability Assay

Cancer cell line proliferation and viability were assessed using the MTT cell viability assay [44]. Briefly, each of the cancer cell lines was seeded at a density of 5 × 10³ cells per well onto 96 well plates. Following this, cell lines were prepared with gradient concentrations of peptide and incubated for 24 h. After this, 10 µL of 5 mg/mL yellow coloured MTT solution (Sigma) were added to all wells and incubated again for 4 h. Once the supernatants were removed by a syringe, 100 µL of DMSO were added to all wells after gently agitating to completely mix the formazan crystals that had developed. A Synergy HT plate reader (BioTek, Winooski, VT, USA) was set at 570 nm for recording the absorbance.