Briefly, tumors were imaged 10 min post 200 μL (15 mg/mL) intraperitoneal injection of D-luciferin (Xenogen Corporation). Isoflurane anesthetized mice were imaged at indicated time points using the Lumina IVIS-100 in vivo Imaging System (Xenogen Corporation). Regions of interest were created and measured as area flux, defined by radiance (photons per second per square centimeter per steradian) according to the manufacturer's calibration (Xenogen Corporation) [19].
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