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The phospho-histone H3 immunofluorescence assay was performed as previously described [49] with primary anti-phoshpo-histone H3 (Cell Signaling #3377) and secondary Alexa Fluor 594 conjugated anti-rabbit (Life Technologies #A-11037) antibodies. Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI). Images were acquired using a Nikon Eclipse Ti microscope with NIS Elements imaging software (Nikon, Melville, NY).
Copyright and License information: ©2016 Shepherd et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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