Statistical analyses were conducted using GraphPad Prism 6.0 software (GraphPad Software, La Jolla, CA) and SAS version 9.4 (SAS Institute Inc., Cary, NC) Group differences in length were analyzed via general linear model, adjusted for age and gender. Data on bone content, histomorphometry and serum IGF-I concentrations were analyzed via an unpaired t test, and results reported as mean values and standard error, with a two-tailed p < 0.05 considered significant. To further evaluate the influence of bone size on the outcomes of bone content, a general linear model included length in addition to age and gender, and where applicable age by group interaction. Additionally, as a sensitivity analysis, models were run adding the use of GoLYTELY in the drinking water.
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